PRR Project

Summary

A highly motivated and dynamic young researcher is needed to develop and apply proteomics approaches and bioinformatics solutions on RISE Thematic Line “Clinical and Translational Research in Cardiovascular Sciences.” RISE aims to strengthen the application of proteomics approaches in cardiovascular research towards the discovery of new therapeutic targets and implementation of new biomarker strategies (multimarker panels and disease signatures) in truly personalized cardiovascular disease management. In this sense, a young researcher with a background in molecular and cellular biology, biochemistry, biomedical sciences, or bioinformatics and experience in proteomics and cardiovascular translational research is required. RISE aims to hire a researcher with experience in sample preparation, method optimization, mass spectrometry analysis, proteome data bioinformatics analysis, and integration with clinical data. A researcher with experience in networking and participation in international projects is preferred to catalyze the position of RISE in the European research environment. Good communication skills, experience in teaching, willingness to apply for national and international funding, to transfer knowledge and invest in proteomics and bioinformatics hard skills are preferred since FMUP also aims to invest in the Continuous Education of their researchers and postgraduation students to improve the competitiveness of the research teams in the application to translational omics projects both nationally and internationally.Main tasks to be performed:Development, application, and optimization of methods for the proteome analysis of biological samples from human and animal models of cardiovascular disease;Sample preparation and analysis of human and animal samples by liquid chromatography/mass spectrometry;Development, application, and optimization of methods for the analysis of proteins posttranslational modifications through liquid chromatography/mass spectrometry;Elaboration of standard operating procedures for the pre-processing, processing, and analysis of cardiovascular biobank samples through omics approaches;Implementation and optimization of methods for the quantification of cardiovascular risk markers in clinical trials led by the faculty;Bioinformatics analysis of omics data, with a focus on proteomics, including functional enrichment analysis, protein-protein interaction analysis, topological network analysis, and prediction analysis;Creation, interpretation, and bioinformatics modulation of hybrid databases, integrating phenomics (clinical data) and molecular data (multi-omics);Correlation, network, and cluster analyses for the identification of putative biomarkers and therapeutic targets;Analysis of omics data in R or other programmatic languages;Molecular biology assays, including western blot, ELISA, and zymography for the validation of surrogate biomarkers and therapeutic targets for cardiovascular diseases, including atherosclerotic disease, aortic valve stenosis, and peripheral artery disease, among others;Assistance in teaching activities in the Master´s and PhD programs in Cardiovascular and Respiratory Sciences, namely in Molecular Biology, Proteomics and Bioinformatics;Organization and lecturing in introductory and advanced courses on multi-omics and bioinformatics for the students, research staff, and other academicians;Supervision of graduate and postgraduate students in translational projects in cardiovascular disease;Application to national and international funding and provision of technical support and expert advice for applications of other faculty members;Public dissemination of the results of translational projects in both national and international conferences, congresses, seminars, and consortia;Preparation of scientific documentation (abstracts, project progress reports, papers).